Comparative study on the HPV infection rate of different esophageal squamous cell carcinoma in Anyang China

VernacularTitle:安阳地区不同食管鳞癌标本HPV感染率的比较研究
Author: Peng QU ¹ ; Jin-Tao LI ; Li-Dong WANG ; Yi ZENG ; Xiu-Sheng CHU
Author Information

1. 山东师范大学
Keywords: Esophageal neoplasms; Carcinoma,sguamous cell; Papilloma virus,human; Infection
From: Chinese Journal of Experimental and Clinical Virology 2012;26(1):34-36
CountryChina
Language:Chinese
Abstract: Objective Comparative and statistical analysis the HPV infection rate between fresh tissue and Paraffin-embedded Specimens of esophageal squamous cell carcinoma,and comparative the testing results with others regions.Methods Extracted the total DNA from the novel fresh tissue and Paraffinembedded Specimens; Detected the DNA by PCR with universal primer and Detected the HPV type with human papilloma virus nucleic acid amplification-based typing detection reagent kit (Hybribio) ; Compared the statistical result from the different specimens; analyzed the result between different region.Results HPV infection rate of fresh tissue is 82.6％ with HPV16 (34.8％ ) and HPV18 ( 34.8％ ),and paraffinembedded specimens is 78.2％ with HPV16 ( 30.4％ ) and HPV18 ( 17.4％ ).Conclusion The results provides the first evidence that there wasn' t noticeable difference between HPV infection rate of the two specimens.So broader specimen source could be used for HPV testing.