The study of an in-house method for drug resistance genotyping testing on HIV-1 strains prevailing in China
10.3760/cma.j.issn.1003-9279.2012.01.023
- VernacularTitle:我国主要HIV-1流行株耐药基因型检测方法的研究
- Author:
Jian-Li NIU
1
;
Hui XING
;
Ling-Jie LIAO
;
Ping ZHONG
;
Peng-Fei MA
;
Yun-Cong WANG
;
Quan-Bi ZHAO
;
Yi-Ming SHAO
Author Information
1. 中国疾病预防控制中心性病艾滋病预防控制中心
- Keywords:
Anti-retroviral therapy;
Viral load;
Genotype;
Polymerase chain reaction
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(1):66-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the amplification rate and the lowestlower detection limit of an in-house HIV-1 Drug resistant (HIVDR) genotyping test.Methods A total of 30 plasma samples were selected,which covered allmajor HIV-1 subtypes predominating prevailing in china (B',CRF07_BC,CRF01 _AE ).The viral loads of the 30 selected samples were detected in triplicate by Easy Q method and the average values were taken as the viral loads of the samples.Each sample was diluted to the concentration of ＞ 1000copies/ml,401-1000 copies/ml,101-400 copies/ml,50-100 copies/ml and ＜ 50 copies/ml with HIV-negative plasma. After extraction of nucleic acids, RT-PCR and nested PCR amplification were performed,the efficiency of amplification of each subtype and the minimum detection limit were determined statistically based on the PCR results.Results The viral loads of the selected samples ranged from 2.03 × 102 - 5.92 × 104 copies/ml.The sample of 50 - 1000 copies/ml have a high amplification rate (86％).Conclusion The In-house method for HIV-1 drug resistance genotyping has a high sensitivity with a high successful amplification rate,especially in the samples with low viral load.This method can be used to the detectionof drug-resistant virus and to provide scientific data to treatment options for patients.
