Characterization of hepatitis B virus antigen expression in vitro and in vivo transduced by different transfer plasmids carrying HBV infectious genome
10.3760/cma.j.issn.1003-9279.2012.02.006
- VernacularTitle:携带乙型肝炎病毒感染性克隆的四种转基因载体在体内外抗原表达分析
- Author:
Xia CHUAI
1
;
Hong CHEN
;
Yang YANG
;
Wen WANG
;
Bo WEN
;
Gang WANG
;
Bao-Ying HUANG
;
Yao DENG
;
Wen-Jie TAN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Hepatitis B virus;
Genome;
Expressed sequence tags;
Hydrodynamic injection
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(2):96-98
- CountryChina
- Language:Chinese
-
Abstract:
Objective To character HBV antigen expression in vitro and in vivo transduced by different transgenic plasmids carrying infectious genome of hepatitis B virus ( HBV ). Methods We constructed four different lentiviral transfer plasmids( carrying 1.3 full-length genome of HBV,by replacing the EGFP express box in pCS-CG plasmid with HBV genome and with different structural element,named as pCS-HBV1.3 (pCS-HBV1.3 X,pCS-HBV1.3 P,pCS-HBV1.3 N and pCS-HBV1.3 K).We detected the expression of HBsAg and HBeAg by ELISA in different time after transfected Huh 7 cells or hydrodynamic injection into C57 BL/6 mice with transfer plasmids pCS-HBV,respectively.Results We detected significant expression of HBsAg over 5 days after transfected Huh 7 cells ( in vitro ) or hydrodynamic injection into C57BL/6 mice(in vivo) with transfer plasmids pCS-HBV1.3 X,pCS-HBV1.3 P and pCS-HBV1.3 K.The expression level and dynamics of HBsAg and HBeAg in the sera of mice is consistent with that of in the supematant of Huh-7 cell.Furthermore,the expression of HBV antigens were modulated by the direction and position of HBV insert,also by some lentivira] vector cis-elements ( cPPT and RRE).Conclusion The optimal lentiviral transfer plasmids (pCS-HBV1.3 X,pCS-HBV1.3 P and pCS-HBV1.3 K) could be further used for establishment and application of HBV transgenic cell or animal model.
