Application of molecularly imprinted technology for separation of PGG from Guizhi Fuling capsule.
- Author:
Ya-ling SONG
;
Xue-jing WANG
;
Fu-yong NI
;
Rui GU
;
Yi-wu ZHAO
;
Wen-zhe HUANG
;
Zhen-zhong WANG
;
Xiao-jie XU
;
Wei XIAO
- Publication Type:Journal Article
- MeSH:
Adsorption;
Capsules;
chemistry;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
Hydrolyzable Tannins;
chemistry;
isolation & purification;
Molecular Imprinting;
Polymers;
chemical synthesis;
chemistry
- From:
China Journal of Chinese Materia Medica
2015;40(6):1012-1016
- CountryChina
- Language:Chinese
-
Abstract:
1,2,3,4,6-penta-O-galloyl-D-glucose (PGG) is one of the main active compounds of Guizhi Fuling capsule. Molecularly imprinted polymers (MIP) have high affinity toward template molecules synthesized by molecularly imprinted technology for its specific combined sites, which can overcome the shortcoming of traditional separation methods, such as complex operation, low efficiency, using large quantity of solvent and environmental pollution. In this paper, surface molecularly imprinted polymer (SMIP) was prepared by surface imprinting with PGG as the template molecule. Its adsorption capacity was measured by the scatchard equation. The separation of PGG from Guizhi Fuling capsule at preparatived scale was achieved with molecularly imprinted polymer as stationary phase and the purity was 90.2% by HPLC. This method can be used to prepare PGG from Guizhi Fuling capsule with large capacity and is easy to operate. It provides a new method for efficient separation and purification for other natural products.
