Effect of celastrol in inhibiting metastasis of lung cancer cells by influencing Akt signaling pathway and expressing integrins.
- Author:
Jia XU
;
Chun-lian WU
;
Jie HUANG
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Line, Tumor;
Drugs, Chinese Herbal;
pharmacology;
Gene Expression Regulation, Neoplastic;
drug effects;
Humans;
Integrins;
genetics;
metabolism;
Lung Neoplasms;
genetics;
metabolism;
pathology;
physiopathology;
Neoplasm Metastasis;
Proto-Oncogene Proteins c-akt;
genetics;
metabolism;
Signal Transduction;
drug effects;
Triterpenes;
pharmacology
- From:
China Journal of Chinese Materia Medica
2015;40(6):1129-1133
- CountryChina
- Language:Chinese
-
Abstract:
Celastrol is a type of quinone methyl triterpene isolated from traditional Chinese medicine Tripterygium wilfordii, with pharmacological activities, like anti-inflammatory, immunosuppression and anti-tumor. This study focused on the effects of celastrol on adhesion, migration and invasion of lung cancer cells. The migration inhibition of lung cancer cells induced by celastrol was detected by the scratch test. The invasion inhibition of lung cancer cells induced by celastrol was measured by the transwell experiment. RT-PCR and Western blot were used to determine the effect of different concentrations of celastrol in integrin family and Akt signaling pathway in lung cancer cells. The results showed that celastrol inhibited adhesion, migration and invasion of lung cancer cells and expressions of integrins β3, β4, αv and phosphorylated Akt, GSK-3β, c-Raf, PDK1 in Akt signal pathway in a dose-dependent manner. Therefore, the study implies that Celastrol could inhibit the metastasis of lung cancer cells by suppressing Akt signaling pathway and expression of integrins.
