Synthesis, identification of artificial antigen of catalpol and preliminary study of immunogenicity.
- Author:
Zhuang LI
;
Wei-ku ZHANG
;
Yue ZHANG
;
Geng-ni DI
;
Hui-hua QU
;
Yan ZHAO
;
Qing-guo WANG
;
Jie-kun XU
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies;
immunology;
Antigens;
chemistry;
immunology;
Immunoassay;
Iridoid Glucosides;
chemistry;
immunology;
Male;
Medicine, Chinese Traditional;
Mice;
Mice, Inbred BALB C;
Serum Albumin, Bovine;
chemistry;
immunology
- From:
China Journal of Chinese Materia Medica
2015;40(7):1287-1290
- CountryChina
- Language:Chinese
-
Abstract:
The method of monoclonal antibody-based immunoassay has a great importance in the study of quality control of traditional Chinese medicine (TCM) and detection of trace components in vivo animals. Synthesis of small molecule artificial antigen is the prerequisite for the establishment of this method. In present study, catalpol-BSA was synthesized by sodium periodate oxidation method. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry ( MALDI-TOF-MS) and molecular exclusion chromatography showed that catalpol was successfully conjugated with BSA. The mice could specifically produce anti-catalpol antibodies with titer up to 1:8000. The artificial antigen of catalpol was successfully synthesized.
