Simultaneous determination of beta-elemene, curcumol, germacrone and neocurdione in volatile oil of Curcuma phaeocaulis and vinegar products by GC-MS.
- Author:
Yan-xiong GAN
;
Ni-ni LUO
;
Yan-ping JIANG
;
Qiao LIU
;
Shu FU
;
Lei WANG
;
Wan LIAO
;
Chao-mei FU
- Publication Type:Journal Article
- MeSH:
Acetic Acid;
chemistry;
Curcuma;
chemistry;
Drugs, Chinese Herbal;
analysis;
isolation & purification;
Gas Chromatography-Mass Spectrometry;
methods;
Oils, Volatile;
chemistry;
isolation & purification;
Plant Oils;
chemistry;
isolation & purification;
Sesquiterpenes;
analysis;
isolation & purification;
Sesquiterpenes, Germacrane;
analysis;
isolation & purification
- From:
China Journal of Chinese Materia Medica
2015;40(7):1311-1315
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to develop a method for determination of beta-elemene, curcumol, germacrone and neocurdione in the volatile oil of Curcuma phaeocaulis, and to provide the basis of the quality control method for the volatile oil of C. phaeocaulis and the related preparations. Based on GC-MS, the 4 main compounds were simultaneously determined, with the internal standard n-tridecane. The Agilent 19091S-433 column (0.25 microm x 250 microm x 30 m) was adopted at the temperature of 250 degrees C, the programmed temperature method (60 degrees C for 1 min, 5 degrees C x min x to 110 degrees C for 5 min, 1 degrees C x min(-1) to 140 degrees C, 5 degrees C x min(-1) to 160 degrees C, 10 degrees C x min(-1) to 240 degrees C) was used. Helium gas was used as the carrier gas at a constant flow rat of 1 mL x min(-1), with an injection volume of 1 RL. Mass spectra were taken at 70 eV; the ion-source temperature was 200 degrees C. The relation time and character acteristic ions for each target compound were determined by full scan mode and SIM, and m/z 85.1, 93.1, 121.1, 107.1 and 180.1 were the detection ions of n-tridecane, beta-elemene, curcumol, germacrone and neocurdione. As a result, beta-elemene, curcumol, germacrone and neocurdione were all detected with good separation. They were all in a good linear relationship within each concentration scope. The average recovery rates were in the range of 98.2%-101%. So, the method can be used to control the quality of the volatile of C. phaeocaulis Val. and the preparations related.
