Rapid identification of chemical composition in safflower with UHPLC-LTQ-Orbitrap.
- Author:
Song-song WANG
;
Yan MA
;
Yi ZHANG
;
De-feng LI
;
Hong-jun YANG
;
Ri-xin LIANG
- Publication Type:Journal Article
- MeSH:
Carthamus tinctorius;
chemistry;
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
Molecular Structure;
Tandem Mass Spectrometry;
methods
- From:
China Journal of Chinese Materia Medica
2015;40(7):1347-1354
- CountryChina
- Language:Chinese
-
Abstract:
The UHPLC-LTQ-Orbitrap high resolution mass spectrometer was used to explore the chemical compositions in safflower. The rapid separation of the compositions was conducted by the UHPLC, following by high resolution full scan and MS2 scan, under the positive and negative ion mode. The chemical formula of compositions were deduced by full scan data in less than 5, then the potential structures were confirmed by the MS2 data. Forty-nine compounds were detected, of which 26 was identified, and 5 compounds was validated by the standard substances.
