Effect of Paridis Rhizoma total saponins on apoptosis of human gastric cancer cell MKN-45 and Fas/FasL signaling pathway.
- Author:
Hai-yan FANG
;
Xiao-yan GONG
;
Xing-hui HONG
;
Ming-liu HUA
;
Jin-ling HUANG
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Caspase 3; genetics; metabolism; Caspase 8; genetics; metabolism; Cell Line, Tumor; Drugs, Chinese Herbal; pharmacology; Fas Ligand Protein; metabolism; Humans; Magnoliopsida; chemistry; Rhizome; chemistry; Saponins; pharmacology; Signal Transduction; drug effects; Stomach Neoplasms; drug therapy; genetics; metabolism; physiopathology; fas Receptor; metabolism
- From: China Journal of Chinese Materia Medica 2015;40(7):1388-1391
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe study aimed to test if Paridis Rhizoma total saponins (PRTS) could induce apoptosis of human gastric cancer cell MKN-45.
METHODBased on the previous researches, PRTS was set by different concentrations to treat human gastric cancer cell for 12 h (5, 10, 20 mg x L(-1)). Fluorescent staining methods were adopted to observe apoptotic morphological changes of MKN-45. The apoptosis rates were analyzed by flow cytometry with Annexin V-FITC/PI staining. The enzymatic activities of caspase-3 and caspase-8 were measured by ELISA. The protein levels of Fas and FasL were detected by Western blotting.
RESULTUnder a fluorescence microscope, MKN-45 treated by PRTS was seen typical apoptotic morphological features. PRTS significantly increased the rate of apoptosis. Compared with the control group, there exsited significant differences in apoptosis rate of PRTS concentration of 20 mg x L(-1) (P < 0.01); besides, the enzymatic activities of caspase-3 and caspase-8 were promoted obviously after the effect of PRTS on MKN-45 cells for 12 h (P < 0.01). The protein levels of Fas and FasL in the MKN-45 were upgraded significantly.
CONCLUSIONPRTS can induce apoptosis of human gastric cancer cell MKN-45 , which is concerned with caspase-3 and caspase-8 and upgraded Fas and FasL.