Isolation, purification and identification of polysaccharides from Gynura divaricata.
- Author:
Zhong-zhen WANG
;
Qian-qian ZHANG
;
Xia HAO
;
Bing LIN
;
Zhi-hong LIU
;
Hong-tao SONG
;
Lei CHEN
- Publication Type:Journal Article
- MeSH:
Asteraceae;
chemistry;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
Magnetic Resonance Spectroscopy;
Molecular Weight;
Polysaccharides;
chemistry;
isolation & purification
- From:
China Journal of Chinese Materia Medica
2015;40(8):1497-1502
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to isolate and purify polysaccharide from Gynura divaricata and analyze its monosaccharide composition. A water-soluble crude polysaccharide was obtained by hot water extraction, ethanol precipitation and deproteinization after degreasing. The crude polysaccharide then purified with DEAE-Sepharose Fast Flow column chromatography and dialysis. The monosaccharide composition and structure were analyzed by HPLC, UV spectrophotometer and 1H-NMR. The results showed that the purity and molecular weight of GDPS-2 and GDPS-3 were 87.3%, 2.03 x 10(4) Da and 90.9%, 4.29 x 10(4) Da, respectively. The UV spectrophotometer and 1H-NMR data suggested that glycosidic bond of GDPS-2 and GDPS-3 were a type. Both GDPs-2 and GDPs-3 were homogeneous polysaccharides, and GDPs-2 was mainly composed of glucuronic acid and xylose at a molar ratio of 1.1:0.63. GDPs-3 was mainly composed of rhamnose, glucuronic acid, galactose, xylose and galacturonic acid at a molar ratio of 0.32:6.0:0.21:1.75:4.3.
