Studies on chromosome numbers of Salvia miltiorrhiza, S. flava and S. evansiana.
- Author:
Hong-xia ZHAO
1
;
Li ZHANG
;
Xing FAN
;
Rui-wu YANG
;
Chun-bang DING
;
Yong-hong ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Chromosomes, Plant; genetics; Diploidy; Plant Roots; genetics; Plants, Medicinal; genetics; Polyploidy; Salvia miltiorrhiza; classification; genetics; Species Specificity
- From: China Journal of Chinese Materia Medica 2006;31(22):1847-1849
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the numbers of chromosome in Chinese herb Salvia miltiorrhiza from 7 provinces in China, and S. flava as well as S. evansiana from Yunnan province in China.
METHODThe young root was treated with the mixture of ice and water for 24 h, fixed with Carony's fixative for 6-12 h. After differentiating for 10-12 min with 1 mol x L(-1) hydrochloric acid at 60 'C and staining with carbol fuchsin,the section was observed under microscope.
RESULTChromosome numbers of S. miltiorrhiza and S.flava were 2n = 2x = 16. The numbers of S. evansiana were 2n = 4x = 32. The basic numbers of the chromosomes were x = 8. And tetraploids were observed in S. miltiorrhiza from Sichuan provices and Shandong provices.
CONCLUSIONThe basic number of the chromosomes are x = 8. The chromosome numbers of S. miltiorrhiza, S.flava and S. evansiana are 16,16 and 32 respectively. As the chromosomes are the small or micro-small ones, it is difficult to use them for karyotype.
