Hairy roots induction of Phellodendron chinense and production of its active constituents.
- Author:
Yue-hua WANG
1
;
Xiao-jun GOU
;
Jie WU
;
Wen-zhao YAN
;
Xian-jun MA
;
Yi YANG
Author Information
- Publication Type:Journal Article
- MeSH: Berberine; analysis; metabolism; Chromatography, High Pressure Liquid; Culture Techniques; methods; DNA, Bacterial; genetics; Phellodendron; genetics; metabolism; Plant Roots; genetics; metabolism; Plants, Medicinal; genetics; metabolism; Rhizobium; genetics; Transformation, Genetic
- From: China Journal of Chinese Materia Medica 2006;31(22):1853-1856
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo introduce the hairy roots of Phellodendron chinense and determine the content of its active constituents.
METHODTransformed hairy roots of P. chinense were obtained by the transformation of Agrobacterium rhizogenes A4, R1600, ATCC15834 and R1000.
RESULT AND CONCLUSIONIt was clearly demonstrated that T-DNA of A. rhizogenes Ri plasmid was integrated into the cells of hairy roots by PCR. The content of berberine hydrochlodride, which was determined by HPLC, was higher in hairy roots than that in the axenic plantet and callus.
