Effects of carbon source and nitrogen source on callus growth and flavonoid content in Glycyrrhiza uralensis.
- Author:
Shi-hai YANG
1
;
Jing TAO
;
Xiao-feng LIU
;
De-an GUO
;
Jun-hua ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Culture Media; pharmacology; Disaccharides; pharmacology; Flavonoids; biosynthesis; Fructose; pharmacology; Glycyrrhiza uralensis; drug effects; growth & development; metabolism; Nitrates; pharmacology; Nitrogen Compounds; pharmacology; Plants, Medicinal; drug effects; growth & development; metabolism; Quaternary Ammonium Compounds; pharmacology; Sucrose; pharmacology; Tissue Culture Techniques
- From: China Journal of Chinese Materia Medica 2006;31(22):1857-1859
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of carbon source and nitrogen source on callus growth, and flavonoid content in Glycyrrhiza uralensis.
METHODInduction and culture of callus were conducted in the media of different concentrations of sucrose, and fructose and ratio of NH4+/NO3- ,and flavonoid content was measured by HPLC.
RESULTThe results showed that fructose was superior to sucrose for callus growth and flavonoid formation, and the optimum concentration was 2%. The flavonoids content was 2 times higher than that of sucrose as carbon sources. In the range of 2% -6% of fructose concentration, the flavonoid content was decreased along with the concentration of fructose, but the licochalcone was increased 5-fold. The highest flavonoid content of 151.47 microg x g(-1) was obtained when the ratio of NH4+/NO3- in the medium was 1/2. NH; inhibited the callus growth and flavonoid formation of G. uralensis.
CONCLUSIONFructose as carbon source was superior to sucrose for callus growth and flavonoid formation, and NO3- was favorable to the callus growth and flavonoids accumulation.
