Study on extraction process of polysaccharide from Sargassum fusiforme by enzymatic treatment.
- Author:
Hua-fang ZHANG
1
;
Jing-shun JIN
;
Rong-mei TAN
;
Cheng SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Cellulase; metabolism; Hydrogen-Ion Concentration; Polysaccharides; analysis; isolation & purification; metabolism; Sargassum; chemistry; Technology, Pharmaceutical; methods; Temperature
- From: China Journal of Chinese Materia Medica 2006;31(22):1860-1862
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the optimal extraction process of polysaccharides from S. fusiforme by enzymatic treatment.
METHODThe optimum extraction conditions were obtained by the experiment with the orthogonal design. The content of polysaccharides of S. fusiforme was determined by spectraphotometry.
RESULTThe amount of enzyme and temperature significantly affected total polysaccharides of S. fusiforme.
CONCLUSIONThe optimum extraction conditions include the addition of 1. 2 x 10 (4) U x 100 g(-1) enzyme into water at pH 4. 5, and the subsequent treatment for 10 min while the temperature is maintained at 45 degrees C.
