Cystic degeneration in liver injury induced by CCl4 in SD rats.
- Author:
Qin XU
1
;
Yi-ping SHEN
;
An-li XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carbon Tetrachloride; Chemical and Drug Induced Liver Injury; Cysts; chemically induced; pathology; Disease Models, Animal; Female; Liver; pathology; Liver Cirrhosis; chemically induced; pathology; Liver Diseases; pathology; Liver Neoplasms; chemically induced; pathology; Male; Precancerous Conditions; chemically induced; pathology; Rats; Rats, Sprague-Dawley; Sex Factors
- From: China Journal of Chinese Materia Medica 2006;31(22):1880-1881
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the hepatic injury induced by CCl4in SD rat.
METHOD40 SD rats were allocated to male and female group, consisting of 20 animals/sex/group. SD rats were given at 2 mL x kg(-1) of 10% CCl4 through celiac injection per 3 day for 12 days. All rats were killed by anaesthesia of ethyl ether and bleeding through abdominal aorta at 12th day. Liver tissue was fixed in 10% neutral formalin, embedded in paraffin, cut at a nominal thickness of 3 microm, stained with hematoxylin and eosin ( H&E) , evaluated at by microscopic examination.
RESULT19 cases with local necrosis, 8 cases with fatty degeneration, 9 cases with cystic degeneration and 2 cases with fibrosis were seen in group male. 20 cases with local necrosis, 9 cases with fatty cases degeneration, 1 case with cystic degeneration and 1 case with fibrosis were seen in group female. The incidence of cystic degeneration in male group was found significantly higher than that in female group (P < 0. 05) , but the incidence of other lesions was no significant difference between male and female group.
CONCLUSIONCCl4 induces local necrosis , fatty degeneration, fibrosis and cystic degeneration in SD rat. The incidences of local necrosis , fatty degeneration and fibrosis were no significantly difference between male and female rat, but the incidence of cystic degeneration in male rats was significant higher than that in female rats.