Simultaneous determination of six main constituents in Swertia of Qinghai Province and Sichuan Province by HPLC.
- Author:
Yi BAO
1
;
Wen-He JI
;
Yu-Hua MA
;
Lan-Ju JI
Author Information
- Publication Type:Journal Article
- MeSH: China; Chromatography, High Pressure Liquid; methods; Glucosides; analysis; Iridoid Glucosides; Iridoids; analysis; Luteolin; analysis; Plants, Medicinal; chemistry; Pyrans; analysis; Pyrones; analysis; Quality Control; Reproducibility of Results; Swertia; chemistry; Xanthones; analysis
- From: China Journal of Chinese Materia Medica 2006;31(24):2036-2038
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a quantitative method of simultaneously determination of swertiamarin, gentiopicroside, mangiferin, swertianolin, isoorientin, 1,8-drihydroxy-3-methoxy-xthanone in Swertia from Qinghai province and Sichuan province by HPLC.
METHODThe samples were separated on the column of Kromasil C18 (4. 6 mm x 250 mm, 5 microm) which eluted with methanol and water (content 0.02% phosphoric acid). The ratio of methanol increased from 20% to 80% during 20-50 min, and from 80% to 100% during 50-60 min, with detected wavelength 254 nm, flow rate at 1 mL x min(-1), column temperature 35 degrees C.
RESULTSix compounds were base-isolated, the linear ranges of swertiamarin, gentiopicroside, mangiferin, 4-swertianolin, 5-isoorientin, 1,8-drihydroxy-3-methoxy-xthanone were excellent.
CONCLUSIONThe method was rapid and precise, and can be use for controlling medicinal materials quality.
