Determination of matrine, sophoridine and oxymatrine in Compound Kushen Injection by HPLC.

Author: Juan TIAN ¹ ; Wei-hao WANG ; Hui-min GAO ; Zhi-min WANG
Author Information

1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Publication Type:Journal Article
MeSH: Alkaloids; analysis; Chromatography, High Pressure Liquid; methods; Drug Combinations; Drugs, Chinese Herbal; chemistry; Quality Control; Quinolizines; analysis; Reproducibility of Results
From: China Journal of Chinese Materia Medica 2007;32(3):222-224
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo establish a high performance liquid chromatography (HPLC) method for determination of matrine, sophoridine and oxymatrine in Compound Kushen Injection.
METHODAlltima amino column was used with acetonitrile-3% phosphoric acid-ethyl alcohol (80:10:10) as the mobile phase and the detection wavelength was at 220 nm.
RESULTThe mean recovery of matrine, sophoridine and oxymatrine in Compound Kushen Injection was 99.51% (RSD 1. 58%), 99.24% (RSD 1.44%) and 100.22% (RSD 1.85%), respectively.
CONCLUSIONThe method was simple, rapid, accurate and specific and suitable for quality control of the product.