Determination of bufothionine in skin of Bufo bufo gargarizans and Huachansu injection.
- Author:
Li-ping DAI
1
;
Zhi-min WANG
;
Hui-min GAO
;
Xu JIANG
;
Guang-zhi DING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bufo bufo; China; Chromatography, High Pressure Liquid; instrumentation; methods; Injections; Materia Medica; administration & dosage; chemistry; isolation & purification; Phenothiazines; analysis; Quality Control; Reproducibility of Results; Skin; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(3):224-226
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a HPLC method for bufothionine in the skin of Bufo bufo gargarizans and Huachansu injection.
METHODThe samples were separated using a Lichrosob C18 column with CH3CN-H2O (10:90) as mobile phase. Flow rate was at 1.0 mL x min (-1) and the detection wavelength was at 225 nm.
RESULTThe calibration curve of bufothionine was linear over the range of 0.0772-0.4632 microg and the average recovery was 99. 2%. The contents of bufothionine were fluctuated from 36.4-641.8 microg x g(-1) in the skin of Bufo bufo gargarizans and 22.47-33.16 microg x mL(-1) in Huachansu injection, respectively.
CONCLUSIONThe contents of bufothionine were greatly different between cultured and wild species. The method was suitable for the quality control of the skin of Bufo bufo gargarizans and its preparation.
