Effects of among compositions of Herba Ephedrae decoction on genic xpression of 5-lipoxygenase activating protein, IL-4 and leukotriene C4 in asthmatic mice.
- Author:
Yong-gang LIU
1
;
Jia-bo LUO
Author Information
- Publication Type:Journal Article
- MeSH: 5-Lipoxygenase-Activating Proteins; Animals; Asthma; chemically induced; genetics; metabolism; Bronchoalveolar Lavage Fluid; chemistry; Carrier Proteins; biosynthesis; genetics; Drugs, Chinese Herbal; isolation & purification; pharmacology; Enzyme-Linked Immunosorbent Assay; Ephedra sinica; chemistry; Interleukin-4; biosynthesis; genetics; Leukotriene C4; metabolism; Lung; drug effects; metabolism; Male; Membrane Proteins; biosynthesis; genetics; Mice; Ovalbumin; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Random Allocation
- From: China Journal of Chinese Materia Medica 2007;32(3):246-249
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the regularity of recipe composition by observing inhibitory effects on the genic expression of 5-lipoxygenase activating protein, IL-4 and the leukotriene C4 in asthmatic mice.
METHODThe mice were challenged with OVA and administered ig with the Herba Ephedrae decoction (HED), separated compositions (2500 mg x kg(-1), calculated by Herba Ephedrae) and dexamethasone (2 mg x kg(-1)) respectively once daily for seven days. The real-time fluorescence quantitative PCR method was employed to measure the contents of FLAP mRNA and IL-4 mRNA expressions in lung and the ELISA method was used to determine the content of LTC4 in the washing solution of pulmonary alveolus and bronchi.
RESULTIn the lung of asthma mice, the expressions of FLAP and IL-4 and the content of LTC4 were significantly augmented compared with the control group. The HED and the separated compositions could suppress the expressions of FLAP and IL-4 and LTC4 release to a great extent in mice.
CONCLUSIONThe HED had the remarkable effects of antianaphylaxis asthma and the original formula HED worked best. These results confirmed the rationality and scientific level of HED.
