Protective action of effective components of Huanglian Jiedu decoction on hypoxia and reoxygenation injury in cultured rat cerebral microvascular endothelial cells.
- Author:
Zheng-zhong YUAN
1
;
Ling-qun ZHU
;
He PANG
;
Ze-song SHAN
;
Shuo-ren WANG
;
Yong-hong GAO
;
Fu-ling NIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Berberine; isolation & purification; pharmacology; Cell Hypoxia; Cell Survival; drug effects; Cells, Cultured; Cerebral Cortex; blood supply; Dose-Response Relationship, Drug; Drug Combinations; Drugs, Chinese Herbal; chemistry; Endothelial Cells; cytology; drug effects; Flavonoids; isolation & purification; pharmacology; Iridoids; isolation & purification; pharmacology; Male; Neuroprotective Agents; pharmacology; Oxygen; pharmacology; Plants, Medicinal; chemistry; Pyrans; isolation & purification; pharmacology; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2007;32(3):249-252
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of geniposide, baicalin and berberine for the rat cerebral microvascular endothelial cell.
METHODThe model of hypoxia and reoxygenation injury in rat cerebral microvascular endothelial cells in vitro was established. Both normal and model cells were treated with geniposide (1.024, 0.512, 0.256, 0.128, 0.064, 0.032, 0.016, 0.008 micromol x mL(-1)), baicalin (0.224, 0.112, 0.056, 0.028, 0.014, 0.007, 0.003 micromol x mL(-1)) and berberine (0.192, 0.096, 0.048, 0.024, 0.012, 0.006, 0.003 micromol x mL(-1)). Cell activity was measured by methyl thiazolyl tetrazolium (MTT) test.
RESULTAfter hypoxia/hypoglycemia cultures for 4 hour and reoxygenation for 12 hour, geniposide (0.128, 0.064, 0.032 micromol x mL(-1)), baicalin (0.028, 0.014, 0.007 micromol x mL(-1)) and berberine (0.024, 0.012, 0.006 micromol x microL(-1) could protect the injuried cerebral microvascular endothelial cells.
CONCLUSIONAppropriate concentration of geniposide, baicalin and berberine, which are effective components of Huanglian Jiedu decoction, could protect the injuried cerebral microvascular endothelial cells.
