Effects and mechanism of lutein on apoptosis of esophageal carcinoma EC9706 cells.
- Author:
Ying-Xin PEI
1
;
Zheng-Chang HENG
;
Guang-Cai DUAN
;
Zun-Zhen ZHANG
;
Ming-Chen WANG
;
Chan-Li HU
;
Dong-Ling GAO
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Carcinoma, Squamous Cell; metabolism; pathology; Cell Line, Tumor; Esophageal Neoplasms; metabolism; pathology; Humans; Lutein; pharmacology; Proto-Oncogene Proteins c-bcl-2; metabolism; bcl-2-Associated X Protein; metabolism
- From: China Journal of Chinese Materia Medica 2007;32(4):332-354
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of lutein on apoptosis and its mechanism.
METHODThe cells of human esophageal carcinoma EC9706 were grown in RPMI medium containing 10% bovine serum and were treated with lutein at 100 microg x mL(-1) concentration. Flow cytometry was employed to investigate the effects of lutein on cell apoptosis of EC9706 cells. Histochemistry was performed to determine apoptosis-related protein expresion.
RESULTFlow cytometry analyses revealed that lutein increased EC9706 cell apoptosis ratio when treated with lutein 100 microg x mL(-1) at 96 h. Lutein decreased the expression of Bcl-2 protein and increased the expression of Bax protein in EC9706 cells.
CONCLUSIONLutein could inhibit mitosis and stimulate apoptosis of EC9706 cells. The apoptotic effect may result from the down-regulation of expression of Bcl-2 and up-regulation expression of Bax.
