Study on separation and purification of total alkaloids and mesaconitine with X - 5 macroporous resin.
- Author:
Wen-Lan LI
1
;
Yan-Ping WANG
;
Yu-Bin JI
;
Yu-Qi FANG
;
Pei ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Aconitine; analogs & derivatives; analysis; isolation & purification; Aconitum; chemistry; Alkaloids; analysis; isolation & purification; Ethanol; chemistry; Hydrogen-Ion Concentration; Plant Roots; chemistry; Plants, Medicinal; chemistry; Resins, Synthetic; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(5):396-400
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study systematically the factors which affect separation and purification of the total alkaloids and mesaconitine with X -5 macroporous resin.
METHODWith the content of the total alkaloids and mesaconitine as parameters, the optimum condition of absorption and elution were studied in the process of the purification with X -5 macroporous resin.
RESULTThe X - 5 macroporous resin yielded the best separating efficiency when the concentration of the extracted solution was 1 g raw material per 1 mL, pH 12.0, the absorptive time of 6 hour and the volume of 95% ethanol (7BV pH 8) as the eluant; X -5 macroporous resins was used five times in a reproducible way. The rate of extraction and content of the total alkaloid were 80% and 30% respectively after purification with X - 5 macroprous resin.
CONCLUSIONThe method can increase the purity of mesaconitine and total alkaloids.
