Effects of glucocorticoid on calcium-activated chloride channel expression in nasal mucosa in allergic rhinitis rats.
- Author:
Wen-li WU
1
;
Hong-tao ZHEN
;
Min GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium Channel Agonists; metabolism; Chloride Channels; metabolism; Dexamethasone; pharmacology; Female; Glucocorticoids; pharmacology; Mucin 5AC; metabolism; Nasal Mucosa; metabolism; Rats; Rats, Sprague-Dawley; Rhinitis, Allergic, Perennial; metabolism
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2009;44(3):242-245
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of CLCA3 and Muc5ac in nasal mucosa in allergic rhinitis rats and the effects of glucocorticoid on its expression.
METHODSThirty SD rats were randomly divided into allergic rhinitis group, dexamethasone group and control group. Expression of CLCA3 mRNA and Muc5ac protein in nasal mucosa were detected by RT-PCR and immunohistochemical assay, respectively.
RESULTSCLCA3 mRNA and Muc5ac protein in allergic rhinitis group were significantly higher than those in control group (t = 8.565, 5.317, P < 0.01, respectively). The increased expression of CLCA3 mRNA in allergic rhinitis group was well correlated with the expression of Muc5ac protein and the correlation coefficient was 0.813 (P < 0.05). After treatment with dexamethasone, the expression of CLCA3 mRNA and Muc5ac protein was notably lower than that in allergic rhinitis group (t = 3.102, 2.226, P < 0.05, respectively).
CONCLUSIONSThe stronger gene expression of CLCA3 exists, complicated with mucus overproduction in the nasal mucosa of allergic rhinitis rats. CLCA3 expression may play a pivotal role in mucus overproduction in allergic rhinitis. Dexamethasone substantially downregulates the expression of CLCA3 mRNA and Muc5ac protein.
