The direct antiviral effect of interferon-gamma on hepatitis C virus replicon.
- Author:
Yin-tang JIA
1
;
Lai WEI
;
Dong JIANG
;
Xu CONG
;
Ran FEI
Author Information
- Publication Type:Journal Article
- MeSH: Antiviral Agents; pharmacology; Female; Hepacivirus; drug effects; Humans; Interferon-alpha; pharmacology; Interferon-gamma; pharmacology; Liver Neoplasms; pathology; Male; RNA, Viral; biosynthesis; drug effects; Replicon; drug effects; Tumor Cells, Cultured; Virus Replication; drug effects
- From: Chinese Journal of Hepatology 2006;14(1):7-10
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of interferon-gamma and interferon-gamma combined with interferon-alpha on HCV RNA replication and the possible mediators of interferon-gamma anti-HCV in vitro.
METHODSAn HCV replicon cell culture system was established and the cells were treated with interferon-gamma or interferon-gamma combined with interferon-alpha. HCV RNA levels in the cells were evaluated by semi-quantitative RT-PCR and real-time PCR, and the levels of NS5A protein were examined by Western blot.
RESULTSInterferon-gamma could inhibit HCV RNA replication and NS5A protein expression effectively; The anti-HCV effects of interferon-gamma were both in time-dependent and does-dependent manners; Pretreating the cells with interferon-gamma could significantly enhance the antiviral effects of interferon-alpha; The expressions of IRF-1, 2'5'-OAS1(p46), 2'5'-OAS2(p69), ISGF3gamma and STAT1 were significantly increased after interferon-gamma treatment.
CONCLUSIONInterferon-gamma has a direct inhibitory effect on HCV replicon RNA replication and NS5A expression, and both are in a dose and time dependent manner; Interferon-gamma has a synergistic anti-HCV effect with interferon-alpha. IRF-1, 2'5'-OAS1(p46), 2'5'-OAS2(p69) and ISGF3gamma may mediate the anti-HCV effects of interferon-gamma.
