Inhibition of HCV replication by HCV specific U1 small nuclear RNA chimeric ribozyme in vivo.
- Author:
Mei-xia WANG
1
;
Qing-long JIN
;
Yu PAN
;
Feng WANG
;
Jun-qi NIU
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Genetic Therapy; Genetic Vectors; Hepacivirus; physiology; Humans; Molecular Sequence Data; RNA, Catalytic; genetics; RNA, Small Nuclear; genetics; Virus Replication; genetics
- From: Chinese Journal of Hepatology 2006;14(1):11-14
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the advantage of U1 small nuclear RNA as a ribozyme vector (U1-Rz) to inhibit HCV replication in vivo.
METHODSThe 3rd stem-loop was substituted by HCV core specific ribozyme to construct an U1-Rz eucaryotic expression plasmid. Then it was co-transfected with pCMV/T7-NCRC Delta-luc into Huh7 cell line mediated by lipofectin. The cell lysis supernatant was subjected to Western blot and lumenometer to determine the luciferase levels.
RESULTSA U1 snRNA chimeric ribozyme was constructed successfully. Both Rz and U1-Rz inhibited luciferase expression in Huh7 by 48.64% and 87.46%, respectively.
CONCLUSIONRz has more efficacy in cells when using U1 snRNA delivery system. U1 can be an efficient vector for HCV specific ribozyme.
