Expression changes of interleukin-1 receptor associated kinase-4 during endotoxin tolerance development in kupffer cells.
- Author:
Sheng-wei LI
1
;
Zuo-jin LIU
;
Chang-an LIU
;
Xu-hong LI
;
Hai-bo YOU
;
Xian-feng CHEN
;
Jian-ping GONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Endotoxins; immunology; Immune Tolerance; Interleukin-1 Receptor-Associated Kinases; biosynthesis; genetics; Kupffer Cells; cytology; immunology; metabolism; Lipopolysaccharides; immunology; Male; Mice; Mice, Inbred BALB C
- From: Chinese Journal of Hepatology 2006;14(2):97-100
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the mechanism of endotoxin tolerance (ET) through observing the expression of interleukin 1 receptor associated kinase-4 (IRAK-4) during endotoxin tolerance development in Kupffer cells (KCs).
METHODSIsolated KCs of Balb/c mouse were divided into two groups: the non-endotoxin tolerance (NET) group and the endotoxin tolerance (ET) group, which were pretreated with 10 ng/ml lipopolysaccharide (LPS) for 24 h. Then, the two groups were treated with 100 ng/ml LPS. The expressions of IRAK-4 gene and protein level were determined by RT-PCR and Western blot. The activities of NF-kappaB of KCs and the TNFalpha level were estimated by ELISA at 0 h, 1 h, 3 h, 6 h and 12 h after LPS stimulation.
RESULTSThe ultimate level of IRAK-4, the activities of NF-kappaB and the TNFalpha level were evidently lower in the ET group than those in the NET group (t = 12.4, 17.4 and 138.9 respectively, P<0.01).
CONCLUSIONSPretreatment with LPS on KCs could induce endotoxin tolerance of KCs and inhibition of IRAK-4 expression may be one of the reasons for its development.
