Influence of repeated seizures and large dosage anti-epileptic drug on phosphorylated cAMP response element binding protein in rat's hippocampus and effect of Caoguo Zhimu Decoction.
- Author:
Juan HE
1
;
Shi-Lei MA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cognition Disorders; chemically induced; prevention & control; Cyclic AMP Response Element-Binding Protein; metabolism; Drugs, Chinese Herbal; therapeutic use; Epilepsy; chemically induced; drug therapy; metabolism; Hippocampus; drug effects; metabolism; pathology; Kindling, Neurologic; drug effects; Male; Pentylenetetrazole; Phenytoin; adverse effects; Phosphorylation; Phytotherapy; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(9):819-821
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the influence of repeated seizures and anti-epileptic drug on phosphorylated cAMP response element binding protein (pCREB) in rat model of cognitive impairment, and the effect of Caoguo Zhimu Decoction (CZD) on it.
METHODSOn the basis of epileptic model induced by pentylenetetrazol (PTZ), cognitive impairment model was induced by kindling epilepsy with PTZ everyday, which were then di-vided into the model-1 group, the CZD-1 group, the nimodipine-1 group, and those by injecting large dosage phenytoin sodium were divided into the model-2 group, the CZD-2 group and the nimodipine-2 group. Changes of pCREB protein in rat's hippocampus were detected using immunohistochemistry and Western blotting assay.
RESULTSThe expression of pCREB was higher in the CZD-1 and nimodipine-1 group than in the model group, while it was significantly different in the CZD-2 and nimodipine-2 groups than in the model-2 group.
CONCLUSIONCZD could relieve the cognitive dysfunction in the epileptic model rats induced by everyday PTZ kindling or by dilantin through increasing the pCREB expression.
