Effect of berberine on nitric oxide release of rat intestinal mucous microvascular endothelial cells.
- Author:
Zhan-Wei SUO
1
;
Ge HU
;
Hui-Qin DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Berberine; pharmacology; Cells, Cultured; Colorimetry; Endothelial Cells; cytology; drug effects; metabolism; Intestinal Mucosa; blood supply; cytology; Nitric Oxide; biosynthesis; Rats; Time Factors
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(9):832-834
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of berberine on the release of nitric oxide (NO) by rat intestinal mucous microvascular endothelial cells (RIMECs) cultured in vitro for exploring the pharmacological mechanism of berberine in treating intestinal disease.
METHODSCultured RIMECs in vitro were identified adopting immunofluorescent stain with factor VIII-related antigen. NO level in the supernatant of normal cell culture or cell culture treated with different concentrations of berberine was detected with colorimetry at the 3rd, 6th, 9th and 12th h and compared.
RESULTSCompared with that in the normal control group, NO level increased more obviously in the berberine treated groups, and the best effect was shown in the 5 microg/mL berberine treated group.
CONCLUSIONOne of the important pharmacological mechanisms of berberine might be through promoting the endogenous NO release to induce endothelium-dependent dilatation of microvascular in intestinal mucosa, thus to improve the local microcirculation of intestine.
