Experimental study on effect of Buchang Naoxintong containing serum for antagonizing hypoxia-induced apoptosis of cortical neurons.
- Author:
Guo-Yu ZHOU
1
;
Xue-Qiang HU
;
Xiao-Hong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Apoptosis; drug effects; Cell Hypoxia; Cell Survival; drug effects; Cells, Cultured; Cerebral Cortex; cytology; DNA Fragmentation; drug effects; Drugs, Chinese Herbal; pharmacology; Female; Male; Neurons; cytology; drug effects; Random Allocation; Rats; Rats, Sprague-Dawley; Serum
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(9):835-838
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Buchang Naoxintong containing serum (BNCS) for antagonizing hypoxia-induced apoptosis of primary cultured cortical neurons with the sero-pharmacological method.
METHODSPrimary cortical neurons from neonate rats (within 24 h) were cultured and induced into hypoxia model on the 7th day, which were then treated with different concentrations of BNCS. Cell apoptosis was detected qualitatively and quantitatively; and further verified by agarose gel electrophoresis through analyzing in-ternucleosomal DNA fragmentation of the neurons. Besides, neuron viability was tested by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and change of nuclear morphology was observed by Hoechst 33258 staining.
RESULTSAfter treatment with BNCS, the viability of the hypoxia neurons improved with significantly reduced neuron apoptosis.
CONCLUSIONBuchang Naoxintong can protect cortical neurons from hypoxia-induced apoptosis.
