Effect of TCM treatment for purging fire, removing toxic substance, relieving stagnation and eliminating phlegm on cytoplasmic free calcium and intercellular adhesion molecule-1 in rats with intracerebral hemorrhage.
- Author:
Kai-pu XIANYU
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium; metabolism; Cerebral Hemorrhage; drug therapy; metabolism; Drugs, Chinese Herbal; therapeutic use; Immunohistochemistry; Intercellular Adhesion Molecule-1; metabolism; Male; Medicine, Chinese Traditional; methods; Neuroprotective Agents; pharmacology; therapeutic use; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(10):919-921
- CountryChina
- Language:Chinese
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Abstract:
OBJECTIVETo study the effects and possible mechanisms of TCM treatment for purging fire, removing toxic substance, relieving stagnation and eliminating phlegm on cytoplasmic free calcium ([Ca2+]i) and intercellular adhesion molecule-1 (ICAM-1) in rats with intracerebral hemorrhage (ICH).
METHODSAdult SD rats were randomly divided into six groups: the normal group, the operated group, the model group, the Naoqing Decoction (NQD) low- and high-dose groups, and the Qingkailing Injection (QKL) group. ICH model was induced by injecting collagenase into right caudate nucleus, level of [Ca2+]i was determined by fluorescent tracing assay, and the expression of ICAM-1 was determined using immunohistochemical technique.
RESULTSHigh- and low-dose of NQD and QKL could significantly antagonize the collagenase injection induced increase of [Ca2+]i (P < 0.01), inhibit the expression of ICAM-1 and shorten its sustainable time.
CONCLUSIONNQD plays a role in protecting neuron by inhibiting calcium overload, regulating cytokines network, and inhibiting inflammatory reaction through postponing the release of cytokines and inflammatory mediators.
