Experimental study on effect of Shumai capsule in promoting angiogenesis in rats with myocardial ischemia.
- Author:
Hui-qiu YIN
1
;
Ji-dong ZHANG
;
Hai-qing LIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Capsules; Coronary Vessels; drug effects; physiology; Drugs, Chinese Herbal; therapeutic use; Female; Gene Expression; drug effects; Male; Myocardial Ischemia; physiopathology; Myocardium; metabolism; pathology; Neovascularization, Physiologic; drug effects; Phytotherapy; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; Vascular Endothelial Growth Factor A; biosynthesis; genetics
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(11):1020-1022
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Shumai Capsule (SMC) on angiogenesis and expression of relevant growth factor in rats with myocardial ischemia (MI).
METHODSModel rats of MI were duplicated and treated with SMC (SMC group), bFGF + calparine (positive control group) and normal saline (model group) respectively. Besides, a sham-operative group was set up and treated with normal saline. The rats were sacrificed in batches at the time after being medicated for 1, 2 and 4 weeks, for determining von Willebrand factor (vWF) and vascular endothelial growth factor (VEGF) protein expression in ischemic myocardium by immuno-histochemical staining, myocardial micro-vessel density (MVD) using digital analysis system, and the gene expression of VEGF by quantitative real-time PCR.
RESULTSCompared with the sham-operative group and the model group, levels of MVD, protein and gene expression of VEGF in the SMC group were higher respectively at three time segments (all P <0.01), but showed insignificant difference to those in the positive control group.
CONCLUSIONSMC could promote angiogenesis in ischemic myocardium of rats, the up-regulation on VEGF mRNA and protein expression might be one of the potential mechanisms of SMC in promoting angiogenesis.
