Overexpression of core binding factor alpha1 enhancing osteoblastic differentiation in adipose-derived stem cells.
- Author:
Xin ZHANG
1
;
Min YANG
;
Lin LIN
;
Ying-fang AO
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Adipose Tissue; cytology; Animals; Cell Differentiation; genetics; Cells, Cultured; Core Binding Factor Alpha 1 Subunit; genetics; metabolism; Genetic Vectors; Male; Osteoblasts; cytology; metabolism; Rats; Rats, Sprague-Dawley; Stem Cells; cytology; metabolism; Transfection
- From: Chinese Journal of Surgery 2008;46(8):610-613
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine whether mouse core binding factor alpha1 (Cbfalpha1) overexpression enhancing osteoblastic differentiation in adipose-derived stem cells (ADSCs).
METHODSADSCs were harvested from SD rats and transduced with the recombinant adenovirus carrying Cbfalpha1 gene. Untransduced cells and cells transduced with adenovirus carrying the enhanced green fluorescence protein (Ad-EGFP) gene served as controls. Cbfalpha1 expression was assessed by immunofluorescence. Alkaline phosphatase (ALP) activity was assayed in 7 and 10 d.
RESULTSOverexpression of Runx2 inhibited adipogenesis, as demonstrated by suppression of LPL expression. Moreover, ADSCs transduced with Ad-Runx2 underwent rapid and marked osteoblast differentiation as determined by osteoblastic gene expression, alkaline phosphatase activity.
CONCLUSIONSCbfalpha1 overexpression enhances osteoblastic differentiation in adipose-derived stem cells.
