Role of silencing PRL-3 expression by miRNA interference in the growth of gastric cancer.
- Author:
Shi-rong CAI
1
;
Zhao WANG
;
Chuang-qi CHEN
;
Ji CUI
;
Chang-hua ZHANG
;
Yu-long HE
;
Wen-hua ZHAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Cell Proliferation; Genetic Vectors; Humans; Lentivirus; genetics; Mice; Mice, Inbred BALB C; Mice, Nude; MicroRNAs; genetics; Neoplasm Proteins; genetics; Protein Tyrosine Phosphatases; genetics; RNA Interference; Stomach Neoplasms; metabolism; pathology; therapy; Transfection; Xenograft Model Antitumor Assays
- From: Chinese Journal of Surgery 2008;46(8):618-621
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of silencing PRL-3 expression by miRNA interference in gastric cancer growth.
METHODSRNA interference mediated by recombinant lentivirus expressing artificial PRL-3 miRNA was employed to knockdown PRL-3 expression in human SGC7901 gastric cancer cells. MTT assay and tumor implantation experiment were conducted to determine the role of PRL-3 in the proliferation of SGC7901 cells and the tumor growth.
RESULTSTransfection of recombinant lentivirus expressing artificial PRL-3 miRNA significantly suppressed the proliferation of SGC7901 cells in vitro. The implanted tumor size of the PRL-3 transfection group was (1.92 +/- 0.18) cm3, significantly smaller than those in control groups [(4.74 +/- 0.39) cm3] (P < 0.05).
CONCLUSIONSSilencing of PRL-3 significantly suppressed the proliferation of SGC7901 cells and tumor growth in vivo. PRL-3 could be a potential therapeutic target in gastric cancer.
