Differential transcription of Bcl-2 and Bax through the cell cycle in prostate cancer cell line.
- Author:
Xue-Song LI
1
;
Kan GONG
;
Guo-Feng SUN
;
Li-Qun ZHOU
;
Zhi-Song HE
;
Dian-Qi XIN
;
Yan-Qun NA
;
Yun-Yan LIANG
;
Dai-Shu WANG
;
Ying-Lu GUO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Cycle; Cell Line, Tumor; Gene Expression; Humans; Male; Prostatic Neoplasms; metabolism; pathology; Proto-Oncogene Proteins c-bcl-2; biosynthesis; genetics; RNA, Messenger; genetics; bcl-2-Associated X Protein; biosynthesis; genetics
- From: Chinese Journal of Surgery 2008;46(10):768-771
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the differential expression of apoptosis associated gene Bcl-2 and Bax through cell cycle and its possible clinical meaning.
METHODSThe prostate cancer cell line PC-3 was synchronized in M, G1, S and G2 phase using modified thymine deoxyriboside blockage and high pressure N2O technique. The efficiency of synchronization was detected by flow-cytometry. RT-PCR and Western blot methods were used to examine the expression of Bcl-2 and Bax in mRNA and protein level.
RESULTSThe synchronized rate of M, G1, S and G2 phase were 92.1%, 87.0%, 80.2% and 75.9% respectively. Bcl-2 was constitutively expressed through the cell cycle, but both the mRNA and protein expression level of Bcl-2 were very high in the G1 phase, dramatically decreased in M, S and G2 phase. The expression level of Bax had no change through the cell cycle.
CONCLUSIONSCell cycle could influence the expression level of Bcl-2 significantly but not Bax, these might have some clinical relevance.
