Construction and identification of small interfering RNA expression plasmid targeting Sox9 and the function to cell growth and apoptosis of human chondrosarcoma cells HTB94.
- Author:
Hong-Min QIN
1
;
Hui-Feng HAN
;
Tie XU
;
Guang-Zhao SHA
;
Lin LIU
;
Yi-Gen PENG
;
Tian-Cheng REN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Proliferation; Chondrosarcoma; metabolism; pathology; Genetic Vectors; Humans; Plasmids; genetics; RNA, Messenger; genetics; RNA, Small Interfering; genetics; SOX9 Transcription Factor; genetics; metabolism; Transfection; Tumor Cells, Cultured
- From: Chinese Journal of Surgery 2008;46(10):772-775
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo construct small interfering (siRNA) Sox9 expression plasmid and transfer it into human chondrosarcoma cells HTB-94, and to check the mRNA and protein expression of Sox9 and cell growth and apoptosis of HTB-94 human chondrosarcoma cells.
METHODSsiRNA(Sox9) expression plasmid was designed and synthesized. And it was transferred into HTB-94 human chondrosarcoma cells. Then the expression of the mRNA and protein of Sox9, cell growth and apoptosis in transferred HTB-94 human chondrosarcoma cells were checked.
RESULTSThe recombinant plasmid was confirmed by enzyme digestion analysis and DNA sequencing. The expression of the mRNA and protein expression of Sox9 in transferred HTB-94 were significantly reduced. The cell growth of HTB-94 was inhibited, and the apoptosis of HTB-94 was remarkably increased.
CONCLUSIONsiRNA (Sox9) expression plasmid could be transferred into HTB-94 human chondrosarcoma cells. And it can reduce the mRNA and protein expression of the HTB-94, inhibit the cell growth and cause the apoptosis of the tumor cells.
