Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage.
10.4142/jvs.2014.15.4.475
- Author:
Li Ming WU
1
;
Rui GUO
;
Lin HUI
;
Yong Gang YE
;
Jing Mei XIANG
;
Chun Yun WAN
;
Miao ZOU
;
Rui MA
;
Xiao Zhuan SUN
;
Shi Jin YANG
;
Ding Zong GUO
Author Information
1. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. vetwu@qq.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Bcl-2;
chronic enteritis;
oxidative damage;
stanniocalcin-1
- MeSH:
Animals;
Animals, Newborn;
Blotting, Western/veterinary;
Caspase 3/*genetics/metabolism;
Cattle;
Cattle Diseases/etiology/*genetics/metabolism;
Duodenum/metabolism;
Enteritis/etiology/genetics/metabolism/*veterinary;
Epithelial Cells/metabolism;
*Gene Expression Regulation;
Glycoproteins/*genetics/metabolism;
Hydrogen Peroxide/pharmacology;
Male;
Proto-Oncogene Proteins c-bcl-2/*genetics/metabolism;
RNA, Messenger/genetics/metabolism;
Real-Time Polymerase Chain Reaction/veterinary
- From:Journal of Veterinary Science
2014;15(4):475-483
- CountryRepublic of Korea
- Language:English
-
Abstract:
Chronic enteritis can produce an excess of reactive oxygen species resulting in cellular damage. Stanniocalcin-1(STC-1) reportedly possesses anti-oxidative activity, the aim of this study was to define more clearly the direct contribution of STC-1 to anti-oxidative stress in cattle. In this study, primary intestinal epithelial cells (IECs) were exposed to hydrogen peroxide (H2O2) for different time intervals to mimic chronic enteritis-induced cellular damage. Prior to treatment with 200 microM H2O2, the cells were transfected with a recombinant plasmid for 48 h to over-express STC-1. Acridine orange/ethidium bromide (AO/EB) double staining and trypan blue exclusion assays were then performed to measure cell viability and apoptosis of the cells, respectively. The expression of STC-1 and apoptosis-related proteins in the cells was monitored by real-time PCR and Western blotting. The results indicated that both STC-1 mRNA and protein expression levels positively correlated with the duration of H2O2 treatment. H2O2 damaged the bovine IECs in a time-dependent manner, and this effect was attenuated by STC-1 over-expression. Furthermore, over-expression of STC-1 up-regulated Bcl-2 protein expression and slightly down-regulated caspase-3 production in the damaged cells. Findings from this study suggested that STC-1 plays a protective role in intestinal cells through an antioxidant mechanism.
