Effects on lymphangiogenesis and angiogenesis of lung cancer with antisense oligonucleotides of vascular endothelial growth factor C.
- Author:
Xu-Feng GUO
1
;
Zhong-Hua XU
;
Yong-Bing CHEN
;
Zhong-Heng XU
;
Wen-Tao YANG
;
Yong-Yue QIAN
;
Guo-Qiang HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Disease Models, Animal; Female; Humans; Liposomes; Lung Neoplasms; metabolism; pathology; Lymphangiogenesis; drug effects; Mice; Mice, Inbred BALB C; Mice, Nude; Microvessels; pathology; Neovascularization, Pathologic; drug therapy; Oligonucleotides, Antisense; pharmacology; Random Allocation; Transfection; Vascular Endothelial Growth Factor C; genetics; metabolism; Xenograft Model Antitumor Assays
- From: Chinese Journal of Surgery 2008;46(9):694-696
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects on lymphangiogenesis and angiogenesis of orthotopic implantation of lung cancer in nude mice with antisense oligonucleotides of VEGF-C.
METHODSThe model in nude mice was established with orthotopic implantation for the human lung cancer cell line A549. Thirty nude mice were randomized into three groups: PBS control group, sense oligonucleotides control group and antisense oligonucleotides group (AODN group). After treatments were completed, the expression of VEGF-C and lymphatic microvessel density (LMVD) and microvessel density (MVD) of lung cancer were detected by RT-PCR,Western Blot and immunohistochemistry.
RESULTSThe expression of VEGF-C in AODN group was inhibit significantly (P < 0.05). The LMVD in AODN group was decreased significantly (P < 0.1). Though the MVD in AODN group was also decreased, but there were no significant differences compared with control groups (P > 0.05).
CONCLUSIONSThe antisense oligonucleotides of VEGF-C can inhibit the expression of VEGF-C in nude mice of orthotopic implantation of lung cancer. It could inhibit the lymphangiogenesis.
