M gene analysis of canine coronavirus strains detected in Korea.
10.4142/jvs.2014.15.4.495
- Author:
Seok Young JEOUNG
1
;
So Yun ANN
;
Hyun Tae KIM
;
Doo KIM
Author Information
1. Department of Veterinary Internal Medicine, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea. kimdoo@kangwon.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
canine coronavirus;
M gene;
phylogenetic analysis
- MeSH:
Amino Acid Sequence;
Animals;
Coronavirus Infections/epidemiology/*veterinary/virology;
Coronavirus, Canine/*isolation & purification;
Dog Diseases/*epidemiology/virology;
Dogs;
Female;
Male;
Molecular Sequence Data;
Phylogeny;
Polymerase Chain Reaction/veterinary;
Republic of Korea/epidemiology;
Reverse Transcriptase Polymerase Chain Reaction/veterinary;
Viral Matrix Proteins/*genetics/metabolism
- From:Journal of Veterinary Science
2014;15(4):495-502
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of this study was to investigate the genetic features of canine coronavirus (CCV) strains detected in Korea. M gene sequences obtained for isolates from 22 dogs with enteritis over a 5-year period were evaluated. Sequence comparison revealed that the 22 Korean CCV strains had an 87.2 to 100% nucleotide homology. Comparing to the typical reference CCV strains (type II), the nucleotide sequence of Korean strains had homology ranged from 86.3% to 98.3% (89.1% to 99.2% for the amino acid sequence) and 87.7% to 97.8% (92.4% to 100% for the amino acid sequence) when compared to FCoV-like CCV strains (type I). Three amino acid variations in the M gene were characteristic for the Korean CCV strains. Phylogenetic analysis demonstrated that the 22 Korean CCV strains belonged to four typical CCV clusters (i.e., a unique Korean CCV cluster, a type II and transmissible gastroenteritis virus cluster, an intermediate cluster between type I and II, and a type I cluster). This study was the first to identify genetic differences of the M gene from Korean CCV strains and provided a platform for molecular identification of different Korean CCV strains.
