Establishing the method of collagen-gel droplet embedded culture drug sensitivity test with rat tail collagen.

Author: Ya HU ¹ ; Tao ZHOU ; Quan LIAO ; Tai-Ping ZHANG ; Ge CHEN ; Yu-Pei ZHAO
Author Information

1. Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Publication Type:Journal Article
MeSH: Animals; Collagen; Drug Screening Assays, Antitumor; methods; Female; Gels; Humans; Male; Rats; Tail; chemistry; Tumor Cells, Cultured
From: Chinese Journal of Surgery 2008;46(13):998-1001
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore the clinical application of collagen-gel droplet embedded-culture drug sensitivity test (CD-DST) in the malignant tumors.
METHODSCD-DST was established with rat tail collagen. Three pancreatic cancer cell lines, surgical resection specimens including 15 cases of pancreatic cancer and 10 cases of gastrointestinal cancer were examined using CD-DST.
RESULTSThe overall achievement ratio of CD-DST for clinical tumor specimens was 80% (20/25). In vitro chemosensitivities of pancreatic carcinoma cells to 5-FU, gemcitabine and oxaliplatin were lower than those of gastrointestinal carcinoma.
CONCLUSIONSCD-DST with rat tail collagen is a valuable chemosensitivity testing method for malignant tumors. It can be used to realize individualized anticancer chemotherapy.