The effects of recombinant human growth hormone on the proliferation of Bel-7402 human hepatic carcinoma cell lines in vitro and its regulation on growth hormone receptors.
- Author:
Jian-Ping LIU
1
;
Tao CHEN
;
Xiao-Xuan CHEN
;
Qing-Jia OU
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; drug effects; Human Growth Hormone; pharmacology; Humans; Liver Neoplasms; metabolism; pathology; Receptors, Somatotropin; genetics; metabolism
- From: Chinese Journal of Surgery 2008;46(13):1006-1009
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of recombinant human growth hormone (rhGH) in the growth of Bel-7402 human hepatic carcinoma cell line (Bel-7402 line) in vitro and its effects on GHR expression.
METHODSTumor cell count, MT assay and colony forming test were performed to determine the responses of Bel-7402 to different concentrations of rhGH (0, 1, 10, 100, 1000, 10 000 ng/ml). Metabolism of DNA in tumor cells was analyzed with the method of mixture of 3H-TdR. Radioreceptor assay was used to detect the GHR expression of the hepatic carcinoma cell lines and its relation to different rhGH concentrations.
RESULTSrhGH accelerated the proliferation of the Bel-7402 line when the concentration of rhGH was over 100 ng/ml (P < 0.05). Other rhGH concentrations had also positive effects, but with reduced effect as compared with that of 100 ng/ml. After 24 h of rhGH addition of concentration of 10 ng/ml and 100 ng/ml, GHR site number was significantly higher than that in control group, while the 10,000 ng/ml group showed a significantly lower GHR site number.
CONCLUSIONSDifferent concentrations of rhGH might result in variable effects on the growth of Bel-7402 hepatic carcinoma cell line. Certain concentrations of rhGH might stimulate the growth of the cell line. rhGH can regulate the expression of GHR in the cell line.
