Genescan analysis of non-small cell lung cancer in the long arm of chromosome 6.
- Author:
Hui GAO
1
;
Qi WANG
;
Baiqiu WANG
;
Chenghui YAN
;
Shengfa WANG
;
Baichun WANG
;
Jiang ZHU
;
Chengbin HUANG
;
Songbin FU
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Non-Small-Cell Lung; genetics; Chromosome Mapping; Chromosomes, Human, Pair 6; Genes, Tumor Suppressor; Humans; Loss of Heterozygosity; genetics; Lung Neoplasms; genetics; Polymerase Chain Reaction; methods
- From: Chinese Journal of Medical Genetics 2002;19(1):14-16
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate if there are microsatellite loci in the long arm of chromosome 6 that have close relationship with non-small cell lung cancer.
METHODSMultiple PCR approach was used to analyze the 18 loci in the long arm of chromosome 6. The PCR products were analyzed in PAGE, and then the electrophoresis maps were analyzed with Gene Scan(TM) and Genotyper(TM).
RESULTSThere were different frequencies of loss of heterozygosity (LOH) in different loci (varying from 3.85% to 38.45%). The total frequency of LOH in 41 gastric cancers was 58.5%(24/41). Eight loci with the LOH frequency higher than 20% were mainly located in 2 regions: 6q24 and 6q27. The accurate location is 6q24-6q25.3 [D6S1699(35%), D6S409(23.33%), D6S441(33.33%)] and 6q26-27 [D6S1550(38.45%), D6S264(20%), D6S1585(25%), D6S446(33.33%), D6S281(30.77%)].
CONCLUSIONThere may be tumor suppressor genes located in the region of 6q24 and 6q27, which have close relationship with non-small cell lung cancer.
