The relationship of p53 gene mutation to cell differentiation and metastasis of laryngeal squamous cell carcinoma.
- Author:
Xiaoqing ZHANG
1
;
Lihong WANG
;
Shixi LIU
;
Xuesong OUYANG
;
Chuanyu LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; genetics; secondary; Cell Differentiation; genetics; Exons; Humans; Laryngeal Neoplasms; genetics; pathology; Lymphatic Metastasis; genetics; Mutation; Neoplasm Staging; Polymerase Chain Reaction; methods; Polymorphism, Single-Stranded Conformational; Sequence Analysis, DNA; methods; Tumor Suppressor Protein p53; genetics
- From: Chinese Journal of Medical Genetics 2002;19(1):61-63
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo inquire about the relationship of p53 gene mutation to the histopathological findings and clinical manifestation in cases of laryngeal squamous cell carcinoma(LSCC).
METHODSThe fresh samples from 60 cases of LSCC were examined. Polymerase chain reaction and silver staining-single strand conformation polymorphism (PCR-SSCP) and DNA direct sequencing were used to detect the mutation of p53 gene in exons 5-8.
RESULTSThe mutation rates were 69.2% and 85.3% in patients at clinical stage I-II and stage III-IV respectively (P>0.05). In the well-, moderately- and poorly-differentiated cell of LSCC, the mutation rates were 52.9%, 83.3% and 94.7% respectively (P<0.05). The p53 gene mutation rate of LSCC patients with neck lymph-node metastasis was 96.4%, whereas that of patients without neck lymph-node metastasis was 62.5% (P<0.05). Twenty samples showed positive results in SSCP; 19 samples showed deletion and mutation in codons 125-292 by DNA direct sequencing.
CONCLUSIONThe mutation of p53 gene in exons 5-8 was closely related to cell differentiation and the neck lymph-node metastasis of LSCC, but it was not related to the clinical stages of the LSCC cases.
