Construction and expression of APP(SWE) transgenic mice model.
- Author:
Wei LIU
1
;
Guangxiu LU
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid beta-Protein Precursor; genetics; Animals; Brain; metabolism; Cloning, Molecular; Female; Gene Expression; Humans; In Situ Hybridization, Fluorescence; Male; Mice; Mice, Inbred C57BL; Mice, Transgenic; Muscles; metabolism; Myocardium; metabolism; Plasmids; genetics
- From: Chinese Journal of Medical Genetics 2002;19(2):104-107
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare transgenic mice model of human Swedish mutation of amyloid precursor protein(APP(SWE)) gene.
METHODSBy pronuclear microinjection, human APP (SWE) genes were microinjected into pronuclei of zygotes of C57 and Kunming white mice. Integral zygotes were implanted into the oviducts of pseudopregnant female mice. The integration and expression of exogeneous genes in offsprings were detected by polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH) and reverse transcription-ploymerase chain reaction (RT-PCR) analysis.
RESULTSThe success rate of microinjection and the birth rate were 76.62% and 10.38%, respectively. There were 6 founders and the integration rate was 35.29%. The exogeneous gene was transferred to the third generation. Expression of human APP(SWE) gene was detected in the brain, heart and muscle but not detected in the liver and kidney of transgenic mice.
CONCLUSIONThe transgenic mice model of human APP(SWE) gene has been prepared successfully.
