In vivo expression of human tissue-plasminogen activator transfected AGZY83-a cells implanted in mice.
- Author:
Yongbo ZHAO
1
;
Feng WANG
;
Yu LI
;
Ying ZHANG
;
Guiyin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Cell Transplantation; methods; Gene Expression; Humans; Mice; Plasmids; genetics; Time Factors; Tissue Plasminogen Activator; blood; genetics; Transfection; Transplantation, Heterologous
- From: Chinese Journal of Medical Genetics 2002;19(2):130-133
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expressing levels of human tissue-plasminogen activator(t-PA) in AGZY83-a cells transfected with pcDNA3.1(+) t-PA in vivo and the feasibility of using transplantation of cells for gene therapy of thrombotic diseases.
METHODSExpression vectors containing the t-PA cDNA gene were transfected into AGZY83-a cells. The transfected AGZY83-a cells were implanted into mice in different regions, and the plasma levels of human t-PA were assayed at intervals.
RESULTSThe plasma levels of human t-PA were significantly increased in mice after implantation of transfected AGZY83-a cells and were significantly higher than those of control groups implanted with untransfected AGZY83-a cells. This significant increase lasted at least 105 days. The intraperitoneal implantation group expressed the highest level of human t-PA, a little higher than that of the subcutis implantation group, while both are much higher than that of the quadriceps femoris implantation group which expressed the lowest.
CONCLUSIONThe implanted transfected AGZY83-a cells are able to stably express high levels of human t-PA, and transplantation of cells transfected with pcDNA3.1(+) t-PA is a new promising method for gene therapy of thrombotic diseases.