Fluorescence probe two-hybridization technique and its application in medical genechips.
- Author:
Yuan YANG
1
;
Sizhong ZHANG
;
Jun WANG
;
Gang YONG
;
Qingjie XIA
;
Shaoqing WU
;
Yan SUN
Author Information
- Publication Type:Journal Article
- MeSH: DNA Probes; chemistry; genetics; DNA, Bacterial; genetics; Fluorescent Dyes; chemistry; Humans; Neisseria gonorrhoeae; genetics; Nucleic Acid Hybridization; methods; Ureaplasma urealyticum; genetics
- From: Chinese Journal of Medical Genetics 2002;19(2):148-151
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a new nucleic acid hybridization detection technique which may be used in medical genechips.
METHODSThe specific DNA fragment was detected by sequential two hybridization of fluorescence probe with template DNA and fixed DNA probe.
RESULTSFluorescence probe two-hybridization (FPTH) was applied to genechips for the detection of sex-transmitted pathogens from culture strains, and the results showed that the values of fluorescence density of the positive groups decreased remarkably when compared with those of the negative group. Both the sensitivity and specificity for detecting clinical samples are higher than 90%. There is no need of any additional reagent in hybridization procedure, and the hybridization detection can be accomplished in 40 minutes.
CONCLUSIONThe FPTH technique is rapid, simple and reliable, it can also make the clinical detection process completely automatic and integrative.
