Linkage analysis of cytokine and cytokine-related receptor gene loci and essential hypertension in Chinese.
- Author:
Shaoli CHU
1
;
Dingliang ZHU
;
Guliang WANG
;
Momiao XIONG
;
Li JIN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Alleles; Blood Glucose; metabolism; Blood Pressure; physiology; Body Mass Index; China; Cholesterol; blood; Cholesterol, HDL; blood; Cholesterol, LDL; blood; Cytokines; genetics; DNA; genetics; Family Health; Female; Genetic Linkage; Humans; Hypertension; blood; genetics; physiopathology; Linkage Disequilibrium; Lod Score; Male; Microsatellite Repeats; Middle Aged; Receptors, Cytokine; genetics; Triglycerides; blood
- From: Chinese Journal of Medical Genetics 2002;19(3):221-224
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the genetic linkage between several cytokine and cytokine-related receptor gene loci and essential hypertension (EH) in Chinese.
METHODSLinkage between seven genetic markers and EH in 95 Chinese nuclear families with EH (including 477 subjects) was analyzed using a technique of fluorescence-based gene scan with DNA short tandem repeat loci. These markers were selected from the chromosomal regions nearby eight cytokines and their receptor genes. The two-point non-parametric linkage analysis (NPL), maximum Lod score and transmission/disequilibrium test (TDT) with GENEHUNTER software package were used in this study.
RESULTSResult of TDT showed significant transmission disequilibrium between D14S61 and EH (Chi square 14.29,P=0.00016) although NPL and Lod score revealed no significant linkage (Z=0.78, P>0.05 and Lod score =0.72 respectively) at this locus. No linkage between other loci typed and EH was found by the three genetic analysis methods (P>0.05 or Lod score<-1).
CONCLUSIONAlleles at D14S61 were of significant transmission disequilibrium in affected siblings. Transforming growth factor beta 3 is 0.1 cM away from D14S61, which suggests that the relationship between genes at or near this regions and EH needs to be further explored.
