Nested coamplification polymerase chain reaction.
- Author:
Qingjie XIA
1
;
Sizhong ZHANG
;
Yuan YANG
;
Jun WANG
;
Xiujie WANG
;
Yongxin MA
;
Yan SUN
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; DNA Probes; chemistry; genetics; Edetic Acid; chemistry; Fluorescent Dyes; chemistry; Microscopy, Fluorescence; Molecular Sequence Data; Nucleic Acid Hybridization; methods; Polymerase Chain Reaction; methods; Reproducibility of Results
- From: Chinese Journal of Medical Genetics 2002;19(4):347-349
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a simple and reliable method for intensifying the hybridization signals of gene chips.
METHODSThe authors added EDTA and another FAM-labeled probe to the normal PCR products, denatured the mixture by heat, and then let the mixture hybridize with the fastened probes on the chip.
RESULTSWith the use of EDTA and another FAM-labeled probe, the hybridization signals increased by 6 times or greater.
CONCLUSIONAdding EDTA and another probe to the normal PCR products is a simple and efficient method to intensify the hybridization signal of chips.
