Preliminary study on the gene expression profiles of oligodendrogliom as with cDNA array.
- Author:
Rongcai JIANG
1
;
Peiyu PU
;
Yu ZHENG
;
Chunsheng KANG
;
Guangxiu WANG
;
Chunyan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Brain Neoplasms; genetics; pathology; Gene Expression; Gene Expression Profiling; Humans; Oligodendroglioma; genetics; pathology; Oligonucleotide Array Sequence Analysis; methods; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Medical Genetics 2002;19(5):379-382
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the gene expression profiles of oligodendrogliomas with gene cDNA array.
METHODS(32)P tagged cDNA probes converted from the total RNA, which had been extracted from 2 fresh samples of oligodendroglioma and 1 of normal brain tissue, were hybridized with the Atlas array. After washing the membranes, the autoradiography was performed and the autoradiograms were analyzed through the special software.
RESULTSAs compared to the normal brain tissue, there were 63 co-upregulated genes and 4 co-downregulated genes in these 2 tumor samples. However, a significant quantitative difference existed between them. The expression trend of some genes differed from the known information.
CONCLUSIONcDNA array is effective for studying the gene expression profiles of oligodendrogliomas and provides new information for the further research on their molecular mechanisms.
