Study on point mutations in mitochondrial DNA control region for replication DLP(6) of cultured dermal fibroblast with 8-MOP/UVA treatment.
- Author:
Zhongrong LIU
1
;
Rongqing LIU
;
Guowei ZHANG
;
Fei HAO
;
Heng YAN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Binding Sites; Cells, Cultured; DNA Replication; DNA, Mitochondrial; drug effects; radiation effects; Dermis; cytology; Fibroblasts; cytology; drug effects; radiation effects; Humans; Male; Methoxsalen; pharmacology; Photosensitizing Agents; pharmacology; Point Mutation; Ultraviolet Rays
- From: Chinese Journal of Medical Genetics 2002;19(5):386-388
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationships between skin photoaging and point mutations in mitochondrial DNA (mtDNA) control region for replication of dermal fibroblast.
METHODSCultured dermal fibroblasts were treated by 8-methoxypsora len /ultraviolet A (8-MOP/UVA). mtDNA was extracted by one-step-method and th e PCR products of D-loop and adjacent transcription promoter (DLP(6)) fragment of mtDNA control region for replication were detected by polymerase chain reaction-single strain conformation polymorphism and direct sequencing.
RESULTSAfter treated by 8-MOP/UVA, point mutations of 414 T-->G of DLP(6) fragment of mtDNA control region for replication largely accumulated.
CONCLUSIONAccumulation of point mutations of DLP(6) fragment of mtDNA control region for replication may be closely associated with skin photoaging.
