Expression of survivin, caspase-3 in oral precancerous lesions and oral squamous-cell carcinoma.
- Author:
Yan-ping DING
1
;
Shu-xia LI
;
Hong-ru WU
;
Xin-yan ZHANG
;
Xiao-fei TANG
;
Zheng SUN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Carcinoma, Squamous Cell; enzymology; metabolism; pathology; Caspase 3; metabolism; Humans; Inhibitor of Apoptosis Proteins; metabolism; Leukoplakia, Oral; enzymology; metabolism; pathology; Mouth Mucosa; enzymology; metabolism; Mouth Neoplasms; enzymology; metabolism; pathology; Precancerous Conditions; enzymology; metabolism; pathology
- From: Chinese Journal of Stomatology 2010;45(2):85-88
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the roles of surviving and caspase-3 in the development of oral cancer.
METHODSArchival tissue sections of 17 oral squamous cell carcinoma (OSCC), 28 oral leukoplakia with dysplasia, 10 normal oral mucosa were obtained from Capital Medical University School of Stomatology for immunohistochemical staining of markers of survivin and caspase-3. The cell apoptosis was detected with terminal deoxynucleotidyl transferase-mediated nucleotide shift enzyme (TdT) mediated d-UTP end labeling (TUNEL). Positively stained cells were counted and analyzed statistically to determine potential relationship between survivin, caspase-3 and cell apoptosis.
RESULTSThe expression of survivin was faint or negative in normal epithelial cells. The average positive rate of survivin was (1.05 ± 1.21)% in control group and (21.89 ± 10.45)% in OSCC. Caspase-3 was expressed in all the normal mucosa,but it obviously down-regulated in dysplasia and OSCC. The apoptosis index (AI) decreased from (0.89 ± 0.46)% in normal mucosa to (0.21 ± 0.12)% in OSCC.
CONCLUSIONSBoth survivin and caspase-3 are associated with carcinogenesis of the oral mucosa. Survivin may restrain cell apoptosis by inhibiting caspase-3.
